550 5.7.54 SMTP; Unable to relay recipient in non-accepted domain

I have a newly installed EX2016 on Azure VM and assigned a public satic IP to it. Updated the MX record and pointed it to the public IP. also, I have opened the port 25 traffic but I am unable to receive an email and send it from my on-prem EX. what cloud be the problem? Error Message:550 5.7.54 SMTP; Unable to relay recipient in non-accepted domain PS: I am learning Exchange



Read more here: https://stackoverflow.com/questions/67018486/550-5-7-54-smtp-unable-to-relay-recipient-in-non-accepted-domain

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